tom misteli Search Results


tom misteli  (ATCC)
92
ATCC tom misteli
Tom Misteli, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tom misteli/product/ATCC
Average 92 stars, based on 1 article reviews
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progerin  (Addgene inc)
90
Addgene inc progerin
(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding <t>for</t> <t>EGFP</t> in the left control hemisphere or ii) <t>progerin</t> in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.
Progerin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/progerin/product/Addgene inc
Average 90 stars, based on 1 article reviews
progerin - by Bioz Stars, 2026-06
90/100 stars
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egfp lamin a d50  (Addgene inc)
93
Addgene inc egfp lamin a d50
(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding <t>for</t> <t>EGFP</t> in the left control hemisphere or ii) <t>progerin</t> in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.
Egfp Lamin A D50, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
egfp lamin a d50 - by Bioz Stars, 2026-06
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pbabe puro gfp progerin plasmid  (Addgene inc)
93
Addgene inc pbabe puro gfp progerin plasmid
(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding <t>for</t> <t>EGFP</t> in the left control hemisphere or ii) <t>progerin</t> in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.
Pbabe Puro Gfp Progerin Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbabe puro gfp progerin plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
pbabe puro gfp progerin plasmid - by Bioz Stars, 2026-06
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lentiviral vector pcdhblast mcsnard ost lmna  (Addgene inc)
91
Addgene inc lentiviral vector pcdhblast mcsnard ost lmna
(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding <t>for</t> <t>EGFP</t> in the left control hemisphere or ii) <t>progerin</t> in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.
Lentiviral Vector Pcdhblast Mcsnard Ost Lmna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviral vector pcdhblast mcsnard ost lmna/product/Addgene inc
Average 91 stars, based on 1 article reviews
lentiviral vector pcdhblast mcsnard ost lmna - by Bioz Stars, 2026-06
91/100 stars
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tom misteli  (Addgene inc)
93
Addgene inc tom misteli
(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding <t>for</t> <t>EGFP</t> in the left control hemisphere or ii) <t>progerin</t> in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.
Tom Misteli, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tom misteli/product/Addgene inc
Average 93 stars, based on 1 article reviews
tom misteli - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
pbabe purogfp lamin a plasmids  (Addgene inc)
93
Addgene inc pbabe purogfp lamin a plasmids
(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding <t>for</t> <t>EGFP</t> in the left control hemisphere or ii) <t>progerin</t> in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.
Pbabe Purogfp Lamin A Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbabe purogfp lamin a plasmids/product/Addgene inc
Average 93 stars, based on 1 article reviews
pbabe purogfp lamin a plasmids - by Bioz Stars, 2026-06
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plenti cmv treg3 neo gfp progerin  (Addgene inc)
93
Addgene inc plenti cmv treg3 neo gfp progerin
(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding <t>for</t> <t>EGFP</t> in the left control hemisphere or ii) <t>progerin</t> in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.
Plenti Cmv Treg3 Neo Gfp Progerin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenti cmv treg3 neo gfp progerin/product/Addgene inc
Average 93 stars, based on 1 article reviews
plenti cmv treg3 neo gfp progerin - by Bioz Stars, 2026-06
93/100 stars
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hp1β gfp  (Addgene inc)
93
Addgene inc hp1β gfp
(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding <t>for</t> <t>EGFP</t> in the left control hemisphere or ii) <t>progerin</t> in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.
Hp1β Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hp1β gfp/product/Addgene inc
Average 93 stars, based on 1 article reviews
hp1β gfp - by Bioz Stars, 2026-06
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pqcxin tetr mcherry retroviral system  (Addgene inc)
93
Addgene inc pqcxin tetr mcherry retroviral system
(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding <t>for</t> <t>EGFP</t> in the left control hemisphere or ii) <t>progerin</t> in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.
Pqcxin Tetr Mcherry Retroviral System, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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Image Search Results


(a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding for EGFP in the left control hemisphere or ii) progerin in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.

Journal: bioRxiv

Article Title: Nuclear aging in polyglutamine-induced neurodegeneration

doi: 10.1101/2023.06.09.544056

Figure Lengend Snippet: (a) Schematic representation of the stereotaxic injection of lentiviruses encoding for mutant ataxin-3 (Mut Atxn3) – injected bilaterally in the striatum of six-week-old C57/BL6 mice - together with i) lentiviruses encoding for EGFP in the left control hemisphere or ii) progerin in the right hemisphere (x: + 0.6; y: +/- 1.8; z: −3.3). Mice were sacrificed at 4 and 8 weeks after surgery, and tissues processed. Detailed images showing immunofluorescence, using anti-human ataxin-3 antibody - 1H9 (Mut Atxn3 intranuclear inclusions in red), EGFP and progerin in green. (b) Immunohistochemical peroxidase staining using anti-DARPP32 antibody as dopaminergic loss of function marker, and depleted volume quantification (c) . Progerin injected hemisphere displayed a higher depletion in DARPP-32 volume when compared with the control hemisphere, 4-week post-injection. (d) Immunohistochemical analysis using anti-ubiquitin antibody and quantification of positive-ubiquitin inclusions, with progerin injected hemisphere displaying a significantly higher number of ubiquitinated inclusions, in 4-week post-injected mice (e) . (f) Cresyl violet staining indicating pyknotic nuclei (orange arrows), and its quantification (g) . Statistical significance was evaluated with paired Student’s t-test (*p < 0.05) n=4 (4 weeks), n=5 (8 weeks). Data are expressed as mean ± SEM. Scale bars represent (b) 500µm, (d) 100µm, and (f) 200µm. DARPP-32 - dopamine and cyclic AMP-regulated neuronal phosphoprotein 32.

Article Snippet: Lentiviral vectors encoding for EGFP (LV-PGK-EGFP), WT Atxn3 (LV-PGK-Atx3 27Q), Mut Atxn3 (LV-PGK-Atx3 72Q) ( ) and Progerin (LMNA HG - pCDHblast MCSNard OST-LMNAd50 – kindly provided by Professor Tom Misteli (Addgene plasmid #22662) ( ) were produced in HEK293T cells with a four-plasmid system, as previously described ( ).

Techniques: Injection, Mutagenesis, Control, Immunofluorescence, Immunohistochemical staining, Staining, Marker, Ubiquitin Proteomics